Non-Primate Monocytes - CD14, CD16 - Ziegler-Heitbrock

Expression of FcmuR by bovine mononuclear blood leukocytes.

Abstract

The receptor for IgM has been identified a few years ago, but its expression by bovine mononuclear cells has not yet been studied. We used rabbit antibodies against bovine FcmuR to begin to fill this gap. Anti-FcmuR antibodies bound to B lymphocytes and monocytes, although less than to neutrophils. Nonclassical and intermediate monocytes (CD172apos CD16pos) bound nonspecifically to rabbit antibodies, complicating analysis, but they bound more anti-FcmuR antibodies than control antibodies, indicating that they also express the FcmuR. They also express more C5a receptors than classical monocytes. Anti-FcmuR antibodies did not bind to CD3pos alphabetaT lymphocytes (both CD4pos and CD8pos) and gammadeltaT cells. At low temperature but not at physiological temperature, purified bovine IgM bound to all monocytes and strongly to all B cells, but hardly to CD3pos T cells. Monocytes and B cells bound human IgA, but IgA did not compete, whereas unlabeled bovine IgM competed for binding of labeled IgM. This supports the role of the FcmuR, and not the FalphamuR, in IgM binding. Finally, we showed that monocytes were able to ingest bacteria opsonized with serum deprived of IgG, indicating their ability to perform IgM-dependent phagocytosis. In conclusion, surface expression of FcmuR by unstimulated blood leukocytes was demonstrated on B cells and monocytes, but not on T cells.