Non-Primate Monocytes - CD14, CD16 - Ziegler-Heitbrock


Three blocks are not enough--Blocking of the murine IgG receptor FcgammaRIV is crucial for proper characterization of cells by FACS analysis.


While binding of IgG antibodies (IgG1, IgG2a, b or c, and IgG3 in the murine system) to Fc gamma receptors (FcγR) via their Fc part is crucial for the activity of therapeutic antibodies in vivo, it is a well-known cause of erroneous results in immunological assays, such as flow cytometric analysis. Thus, blocking of the IgG-binding sites of FcγRs has been established as a preventive procedure.

Authors: Biburger M, Trenkwald I, Nimmerjahn F
Journal: Eur. J. Immunol.; 2015 Sep; 45(9) 2694-7. doi:10.1002/eji.201545463
Year: 2015
PubMed: PMID: 26138319 (Go to PubMed)