Non-Primate Monocytes - CD14, CD16 - Ziegler-Heitbrock

Three blocks are not enough--Blocking of the murine IgG receptor FcgammaRIV is crucial for proper characterization of cells by FACS analysis.

Abstract

While binding of IgG antibodies (IgG1, IgG2a, b or c, and IgG3 in the murine system) to Fc gamma receptors (FcγR) via their Fc part is crucial for the activity of therapeutic antibodies in vivo, it is a well-known cause of erroneous results in immunological assays, such as flow cytometric analysis. Thus, blocking of the IgG-binding sites of FcγRs has been established as a preventive procedure.