Non-Primate Monocytes - CD14, CD16 - Ziegler-Heitbrock


Nr4a1-dependent non-classical monocytes are important for macrophage-mediated wound healing in the large intestine.


Introduction: Macrophages play an important role in intestinal wound healing. However, the trajectories from circulating monocytes to gut macrophages are incompletely understood. Methods: Taking advantage of mice depleted for non-classical monocytes due to deficiency for the transcription factor Nr4a1, we addressed the relevance of non-classical monocytes for large intestinal wound healing using flow cytometry, in vivo wound healing assays and immunofluorescence. Results: We show that wound healing in Nr4a1-deficient mice is substantially delayed and associated with reduced peri-lesional presence of macrophages with a wound healing phenotype. Discussion: Our data suggest that non-classical monocytes are biased towards wound healing macrophages. These insights might help to understand, how targeting monocyte recruitment to the intestine can be used to modulate intestinal macrophage functions.

Authors: Heidbreder K, Sommer K, Wiendl M, Müller TM, Atreya I, Hildner K, Neurath MF, Zundler S,
Journal: Front Immunol;2023 Jan 18;13:1040775 doi:10.3389/fimmu.2022.1040775
Year: 2023
PubMed: PMID: 36741412 (Go to PubMed)